Endocrine Abstracts

Tumour necrosis factor-alpha (TNF-α) is a pleiotropic cytokine well characterised as a mediator of skeletal muscle wasting. However, the role of interlukin-6 (IL-6) in skeletal muscle remains controversial.Objectives: We therefore investigated the potential interactive effects of TNF-α and IL-6 on murine C2 skeletal myoblast survival, differentiation and proliferation.Methods: Real time-PCR, creatine kinase assay, western...

Introduction: Prolonged elevations of proinflammatory cytokines are associated with sarcopenia muscle wasting (ageing) and in cancer cachexia. Increased activation of the IGF/insulin pathway is an attractive target for combating such wasting.Aims: Using rodent skeletal muscle cell lines we have investigated TNF-α/IGF-I interactions, in an attempt to mimic and understand mechanisms underlying skeletal muscle loss.Methods and re...